The virtual course "Basic concepts of extracellular vesicles - Virtual Course - Coursera", is a course with different contents and offers video classes of Approx. 10 hours to complete. Explore its essential features, and click the orange button for detailed information on the Coursera e-Learning platform.
This course aims to provide basic knowledge about extracellular vesicles (EVs), a generic term that includes exosomes, microvesicles, microparticles, ectosomes, oncosomes, prostasomes, and many others. It covers areas such as the history of electric vehicles, nomenclature, biogenesis, electric vehicle charging, as well as release and absorption mechanisms, collection and processing before isolation, different isolation methods, techniques of characterization and quantification. This course is divided into five modules. Module 1 is an introduction to the field and will cover the nomenclature and history of electric vehicles. Module 2 will focus on the biogenesis, release and uptake mechanisms of EVs, as well as the different charges of EVs (RNA, proteins, lipids). In Module 3, we will focus on the collection and processing of cell culture media and body fluids such as blood, breast milk, cerebrospinal fluid, and urine prior to isolation from EVs. Modules 4 and 5 will present different isolation methods and characterization/quantification techniques, respectively. Differential ultracentrifugation, size exclusion chromatography, density gradient, kit-based precipitation, electron microscopy (EM), cryo-TEM, flow cytometry, atomic force microscopy, and nanoparticle tracking analysis will be presented here. Recommended prerequisites are college level biology and biochemistry. After completing a course, you should be able to: + Discuss the nomenclature and subgroups of extracellular vesicles. + Describe the RNA, protein and lipid content of extracellular vesicles. + Describe the basics of the most common isolation and characterization techniques and how these techniques are used in the field of electric vehicles. + Indicate the benefits and limitations of the most common techniques for the isolation and characterization of extracellular vesicles. + Explain the considerations that are important during the collection and isolation of electric vehicles from different body fluids. + Describe the mechanisms of release and uptake of extracellular vesicles. All lectures are given in English. Each of the five modules will be followed by an exam. All exams will be in the multiple-choice question format. The course is organized in collaboration between the International Society for Extracellular Vesicles (ISEV), the University of California Irvine (USA). USA), the University of Gothenburg (Sweden) and the Pohang University of Science and Technology (South Korea). + Explain the considerations that are important during the collection and isolation of electric vehicles from different body fluids. + Describe the mechanisms of release and uptake of extracellular vesicles. All lectures are given in English. Each of the five modules will be followed by an exam. All exams will be in the multiple-choice question format. The course is organized in collaboration between the International Society for Extracellular Vesicles (ISEV), the University of California Irvine (USA). USA), the University of Gothenburg (Sweden) and the Pohang University of Science and Technology (South Korea). + Explain the considerations that are important during the collection and isolation of electric vehicles from different body fluids. + Describe the mechanisms of release and uptake of extracellular vesicles. All lectures are given in English. Each of the five modules will be followed by an exam. All exams will be in the multiple-choice question format. The course is organized in collaboration between the International Society for Extracellular Vesicles (ISEV), the University of California Irvine (USA). USA), the University of Gothenburg (Sweden) and the Pohang University of Science and Technology (South Korea). All exams will be in the multiple-choice question format. The course is organized in collaboration between the International Society for Extracellular Vesicles (ISEV), the University of California Irvine (USA). USA), the University of Gothenburg (Sweden) and the Pohang University of Science and Technology (South Korea). All exams will be in the multiple-choice question format. The course is organized in collaboration between the International Society for Extracellular Vesicles (ISEV), the University of California Irvine (USA).
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